| CASP8

Caspase-8 is a caspase protein, encoded by the CASP8 gene. It most likely acts upon caspase-3. CASP8 orthologs have been identified in numerous mammals for which complete genome data are available. These unique orthologs are also present in birds.The CASP8 gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	10	5	2.5	1.25	0.62	0.31	0.15	0
OD	2.59	1.47	0.81	0.48	0.33	0.23	0.21	0.08
Corrected OD	2.51	1.39	0.73	0.4	0.25	0.15	0.13	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse CASP8 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Cell Culture Supernates(n=8)	96-108	104

 

| Linearity

Samples were spiked with high concentrations of Mouse CASP8 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Cell Culture Supernates(n=4)
1 : 2	Range (%)	90-110
	Average (%)	96
1 : 4	Range (%)	105-115
	Average (%)	108